Automatic microtubule tracking in fluorescence images of cells doped with increasing concentrations of taxol and nocodazole

Abstract

The purpose of this paper is to provide an algorithm for detecting and tracking astral MTs in a fully automated way and supply a description of their dynamic behaviour. For the algorithm testing, a dataset of stacks (i.e. time-lapse image sequences), acquired with a confocal microscope, has been employed. Cells were treated with two different drugs, nocodazole and taxol, in order to explore their effect on microtubule dynamic instability.