Fast fluorescence dynamics in non-ratiometric calcium indicators
arXiv:0807.2756 · doi:10.1364/OL.34.000362
Abstract
A fluorescence decay of high-affinity non-ratiometric Ca2+ indicator Oregon Green BAPTA-1 (OGB-1) is analyzed with unprecedented temporal resolution in the two-photon excitation regime. A triple exponential decay is shown to best fit the fluorescence dynamics of OGB-1. We provide a new model for accurate measurements of the free Ca2+ concentration and dissociation constants of non-ratiometric calcium indicators.
3 pages, 2 figures, figures revised, added chi-square goodness of fit